Receptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee

The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified certain plants as candidates for ( R ) genes or membrane receptors that activate genes. RGAs different possess conserved domains play specific roles fight against Despite importance RGAs, these molecular disease are still unknown. This study aimed sequence characterize candidate from with potential involvement H. Sequencing was performed based on a library bacterial artificial chromosomes (BAC) clone ‘Híbrido de Timor’ (HdT) CIFC 832/2 screened using functional marker. Two HdT_LRR_RLK1 HdT_LRR_RLK2, containing motif leucine-rich repeat-like kinase (LRR-RLK) were identified. Based presence absence HdT_LRR_RLK2 RGA number differential clones combinations gene, did not correspond any already characterized (SH1-9). These also analyzed qPCR demonstrated major expression peak at 24 h after inoculation both compatible incompatible interactions between results valuable information breeding programs developing CLR-resistant cultivars, addition enabling better understanding